Incorporation of [2-(sup)3H]glycerol into cell surface components of Bacillus subtilis 168 and thermosensitive mutants affected in wall teichoic acid synthesis: effect of tunicamycin
Article Abstract:
Research demonstrates that wall teichoic acid is a growth-inhibitory target for tunicamycin antibiotic in Bacillus subtilis. Data further show that at higher concentrations, the antibiotic inhibits wall teichoic acid synthesis.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2000
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In Bacillus subtilis 168, teichoic acid of the cross-wall may be different from that of the cylinder: a hypothesis based on transcription analysis of tag genes
Article Abstract:
The formation of the teichoic acid, poly(glycerol phosphate) (poly(groP)), in Bacillus subtilis 168 is controlled by genes present in the tagAB and tagDEF operons, whose transcription is different during different phases of growth. The transcription of tagD and tagAB increases as the growth progresses from the exponential phase to the stationary phase. However tagD is expressed more than tagA. The tagE and tagF genes are not transcribed during the exponential growth phase. This difference in expression of these genes changes the amount of poly(groP) formed during cross wall and cylinder wall formation.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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Assay for UDPglucose 6-dehydrogenase in phosphate-starved cells: gene tuaD of Bacillus subtilis 168 encodes the UDPglucose 6-dehydrogenase involved in teichuronic acid synthesis
Article Abstract:
A study was conducted to characterize a new assay permitting the detection of UDPglucose 6-dehydrogenase activity in cell-free extracts determined from phosphate-starved cultures of Bacillus subtilis. Results indicated the presence of an enzymic activity oxidizing UDPglucose to UDPglucuronic acid in the cytoplasm. Findings also showed that the detection of the activity in whole-cell-free extracts were hindered by the presence of periplasmic enzymes catalyzing the degradation of the sugar nucleotides.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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